Effects of natural and recombinant interferon on carbon tetrachloride-induced liver cirrhosis

Carbon tetrachloride [CCl[4]] is a well-known hepatotoxicant that is used to develop a model of liver cirrhosis in rats. The present study was conducted to investigate the effects of chronic treatment with natural or recombinant interferon [IFN], Ismafron or Roferon, respectively, on CCl[4]-induced liver cirrhosis in rats. Seventy male Sprague-Dawley rats were used in the current study; animals were divided into three major groups, Group I, non-treated animals; Group II, normal animals treated with Ismafron, Roferon or liquid paraffin [the vehicle of CCl[4]]; Group III, cirrhotic animals; the cirrhotic rats were divided into 3 subgroups, cirrhotic animals treated with Ismafron, cirrhotic animals treated with Roferon, and untreated cirrhotic animals. Liver cirrhosis was induced in rats after 10 weeks of treatment with CCl[4] at a dose of 0.3 ml/100 g body weight twice a week. Two weeks of treatment with Ismafron [10,000 IU/rat/day] or Roferon [50,000 IU/rat/day] could decrease the serum activities of the enzymes alanine aminotransferase [ALT], alkaline phosphatase [ALP], and gamma-glutamyl transferase [GGT], and suppress the serum level of procollagen III. Furthermore, treatment with Ismafron or Roferon decreased fibrosis and inflammation of cirrhotic livers. It is concluded that treatment with Ismafron or Roferon could normalize the above serum markers of liver cirrhosis and enhance the histological picture of the liver in CCl[4]-treated rats

Some haematological studies on marshal in white rats

Forty rats were used to investigate the effects of marshal insecticide on hemogram, bone marrow and blood coagulation. Treated rats were fed on a ration containing 500 ppm marshal for 3 weeks. Blood and bone marrow samples were taken weekly and subjected to examination of RBCs and WBCs count, Hb concentration, PCV, platelet count, fibrinogen, PT and APTT. In addition to determination of M/E ratio in the bone marrow. The same tests were done in the control group. A marked decrease in RBCs count, Hb concentration and PCV was noted with development of microcytic hypochromic anaemia. Bone marrow showed a marked increase in the M/E ratio. A significant decrease in platelet count was recorded while fibrinogen values, PT and APTT were significantly increased throughout the experiment

Some Toxicological studies on sodium fluoride in clarias lazera

Clarias lazera was exposed to 75 mg/l sodium fluoride for 3 weeks. Blood and serum samples were collected weekly and subjected to haematological and biochemical examination. Muscle samples were also taken during the exposure period and during withdrawal of NaF for 2 weeks. PT and APTT showed significant increase in their values while platelet count was within the normal limits. Total protein, albumin and urea values were markedly increased while ALT, globulin and creatinine showed insignificant changes. Fluorine ion concentration ranged from 5.719 +/- 0.239 to 6.129 +/- 0.339 ppm in the exposure phase the decreased to be 5.631 +/- 0.135 ppm at the end of withdrawal phase

Wound healing-promoting effect of topically applied honey: a comparative experimental study with madecassol and solcoseryl

The present study was carried out on 120 rats to evaluate the role played by commercial bee honey in promoting wound healing as compared to madecassol and solcoseryl. Measurements of residual bare are left at the end of 5, 10 and 15 days following the surgical excision of full thinkness circular 12 mm piece of nape skin showed a reduction by 29%, 10.3% and 17% respectively, as a result of specific healing promoting action of honey. Reduction in wounds of the other groups was less consistent. Honey is cheap, accessible and a simple remedy for wound healing and as nutritions biological product it can be efficient under wide range of wound conditions